Solutions and reagents:
50 mM Tris-HCI
50 mM Tris
For 1 L:
6 g Tris base(MW: 121.14 g)
100 mM HCI
pH (at 25℃)
45.7 mL
7.1
44.7 mL
7.2
43.4 mL
7.3
42.0 mL
7.4
40.3 mL
7.5
38.5 mL
7.6
36.6 mL
7.7
34.5 mL
7.8
32.0 mL
7.9
29.2 mL
8.0
26.2 mL
8.1
22.9 mL
8.2
19.9 mL
8.3
17.2 mL
8.4
14.7 mL
8.5
12.4 mL
8.6
10.3 mL
8.7
8.5 mL
8.8
Add distilled water to a final volume of 1 L.
TBS (concentrated Tris-buffered saline)
20 mM Tris
150 mM NaCl
For 1 L 10x storage buffer:
24 g Tris base (MW: 121.14 g)
88 g NaCl (MW: 58.4 g)
Dissolve in 900 mL distilled water
pH to 7.6 with 12 N HCl
Add distilled water to a final volume of 1 L.
For a 1x solution, mix 1 part of the 10x solution with 9 parts distilled water and adjust pH to 7.6 again.
TBST (Tris-buffered saline, 0.1% Tween 20)
For 1 L:
100 mL of TBS 10x storage buffer
1 mL Tween 20
Add distilled water to a final volume of 1000 mL.
0.1 M Gly-HCl
7.507 g Glycine
1.6 mL HCl
Add distilled water to a final volume of 1 L.