Dilution Calculator
Calculate the dilution required to prepare a stock solution
The dilution calculator equation
The dilution calculator is based on the following equation:
Concentration(start) x Volume(start) = Concentration(final) x Volume(final)
This equation is commonly abbreviated as: C1V1 = C2V2
An example of a dilution calculation using the dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
1.Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
2.Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
3.Enter 20 into the Volume (final) box and select the correct unit (milliliter)
4.Press calculate
5.The answer of 100 microliter (0.1 ml) appears in the Volume (start) box
Reconstitution Calculator
Calculate the volume of solvent required to reconstitute your vial
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
Molarity Calculator
Calculate the mass, volume, or concentration required to prepare the solution
The molarity calculator is a useful tool which allows you to calculate the:
● mass of a compound required to prepare a solution of known volume and concentration
● volume of solution required to dissolve a compound of known mass to a desired concentration
● concentration of a solution resulting from a known mass of compound in a specific volume
*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).
The molarity calculator is based on the following equation:
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
1.Enter 197.13 into the Molecular Weight (MW) box
2.Enter 10 into the Concentration box and select the correct unit (millimolar)
3.Enter 10 into the Volume box and select the correct unit (milliliter)
4.Press calculate
5.The answer of 19.713 mg appears in the Mass box
